Insertion junction: CLIP2.078743_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.078743
Chromosome:plastome
Location:80150
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802297 psbB,2717002,ChreCp032 (1 of 1) K02704 - photosystem II CP47 chlorophyll apoprotein (psbB); photosystem II P680 chlorophyll A apoprotein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCAATACTACCCATTGATAAACCGAAGTATAAACGAATAGAAGGACG
Internal bar code:CTTCAACTACGTCTTCGCTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:362
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGGCAGGTTCTATGGCT
Suggested primer 2:TGTTGGCATACGACGAACGA