| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078752 |
| Chromosome: | chromosome 17 |
| Location: | 5613795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739900 | (1 of 1) IPR012337//IPR027417 - Ribonuclease H-like domain // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACCTAATCTCCCCCCTACCGACGTTCCTTCCCGGCGCTGCCTCGGTC |
| Internal bar code: | TGGATAGGCGATTAGTATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5426 |
| LEAP-Seq percent confirming: | 86.1538 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCCATCTGCTGCCTTG |
| Suggested primer 2: | ACGTACCGCATCCATCCATC |