Insertion junction: CLIP2.078757_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.078757
Chromosome:chromosome 3
Location:5736611
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre03.g187200 LC6,ODA13,DLL2,ODA-LC6 (1 of 2) K10418 - dynein light chain LC8-type (DYNLL); Outer Arm Dynein Light Chain 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGGGAGTGAACGGAGTGTCCTTTGATTTCCATAGGATGACGTGGTACG
Internal bar code:CCAAAGTTCGCCTGATGATGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2243
LEAP-Seq percent confirming:44.6429
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTCCAAATAGCGCTCA
Suggested primer 2:TGCCCTTCCCTTTGTTGGTT