Insertion junction: CLIP2.078761_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078761
Chromosome:chromosome 3
Location:6043329
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTGAGTGCTATTGCGTACTGCTTTACTTTAGACAGGGCTGAAGTTTT
Internal bar code:AATCCATTGTAATCAGGCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1678
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCGCTACAGGATCTGAG
Suggested primer 2:TATCCAGAGAACCCCGCTGA