| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078767 |
| Chromosome: | chromosome 5 |
| Location: | 3479081 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233450 | ELG30 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 30 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCGGGCGCGGCCTCGCCGGAGCAGCACGAGGAGTCGGCGCTGGACC |
| Internal bar code: | ACCTTAAATCTGCAAATTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 70.3704 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCCATCTTTGCAACCC |
| Suggested primer 2: | ATGCATGGAGGACACCACAG |