| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078769 |
| Chromosome: | chromosome 2 |
| Location: | 3691085 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100350 | (1 of 2) PTHR31350:SF1 - F-BOX ONLY PROTEIN 21 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGACTCAGGTGGTTGGCGTCGTACAACCGCTCAACCAACAACGATC |
| Internal bar code: | TTGTAATCTCTTAGCCCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGAGACTCGGGAGGT |
| Suggested primer 2: | ATGCCGACAGTTTCAGGAGG |