| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078772 |
| Chromosome: | chromosome 6 |
| Location: | 1903588 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264200 | SDH2 | (1 of 1) K00235 - succinate dehydrogenase (ubiquinone) iron-sulfur subunit (SDHB, SDH2); Iron-sulfur subunit of mitochondrial succinate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCAGACCCCTGCCTTTCGTATCCGTGTGGCTACCGGTAGGCCAGTA |
| Internal bar code: | TACCTTGTGATGTGCGATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2035 |
| LEAP-Seq percent confirming: | 17.1429 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCAGTCTCTGGCCAAG |
| Suggested primer 2: | GGTTGTGGTTGTCGAACTGC |