Insertion junction: CLIP2.078773_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.078773
Chromosome:chromosome 2
Location:5750513
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g116750 ATP1A Mitochondrial F1F0 ATP synthase, alpha subunit; (1 of 1) K02132 - F-type H+-transporting ATPase subunit alpha (ATPeF1A, ATP5A1, ATP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGCGCACACCCGCGACGCCACCTTGTCCCGGACTCACCCGCGCGGGC
Internal bar code:TCGCACAGCGCTGAATGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACAGGGAGGGCTGAGAT
Suggested primer 2:CAGTCAAGCGCAAACGTTGA