Insertion junction: CLIP2.078784_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.078784
Chromosome:chromosome 9
Location:6313365
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g414000 VTE8,HPT1 (1 of 1) 2.5.1.116 - Homogentisate geranylgeranyltransferase / HGGT; homogentisate phytyltransferase/ prenyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGGTCCGTCCCACCGCCACGACAGATGTCACACCGCCACTGTCGGGGA
Internal bar code:ACAGTAAGTGGCGGGGTCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3169
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTCCTCGATACGCATGG
Suggested primer 2:GAGTTGCACAAGTAGGGGCT