| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078790 |
| Chromosome: | chromosome 1 |
| Location: | 1607884 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACGCACCCTACTCTGCACGGCCTGGGCCCTGCCCTGCCTCCGCAGTTT |
| Internal bar code: | CTCTGGAGTTCCGCAATGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 32.7869 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCAACACCAGCACAAGA |
| Suggested primer 2: | CGACTGCGTTTGTGACTGTG |