| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078795 |
| Chromosome: | chromosome 10 |
| Location: | 739950 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422600 | NUO6 | NADH:ubiquinone oxidoreductase 51 kDa subunit; (1 of 1) K03942 - NADH dehydrogenase (ubiquinone) flavoprotein 1 (NDUFV1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAGGTGGTGGAAAGAGCAAACAGCATTCAGGGCGCATGCATGTGCAC |
| Internal bar code: | TTGGACTCTGATCCATAGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCCTCTCTGCAGTATCT |
| Suggested primer 2: | TGAAACCGAGTCCACGTCTG |