Insertion junction: CLIP2.078799_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078799
Chromosome:chromosome 11
Location:41228
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467526 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTACACACTATAGGAGGAACGACTGCGTCGGTCCAGGCTTGCATCG
Internal bar code:GGCCTCGCGTAGTCCCGGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3957
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGATACCGGCATGACTC
Suggested primer 2:GGCTGTTGACAACTGTGCTG