| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.078805 |
| Chromosome: | chromosome 1 |
| Location: | 3316746 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021400 | SPL13 | Pre-mRNA splicing factor; (1 of 1) PTHR16196//PTHR16196:SF0 - CELL CYCLE CONTROL PROTEIN CWF25 // PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG | 3'UTR |
| Cre01.g021450 | TPR4 | Tetratricopeptide-repeat protein 4; (1 of 1) PTHR23083//PTHR23083:SF443 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACCTTAGCGGGCGCTTGCAGGCAAATTGTTGTGTAATCTGTATACG |
| Internal bar code: | GGCGCAACGTAGTTATGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1344 |
| LEAP-Seq percent confirming: | 47.3684 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTTGCGGTAGAGCTGT |
| Suggested primer 2: | GGAAGGAGTACAAGACCGGC |