Insertion junction: CLIP2.078812_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078812
Chromosome:chromosome 14
Location:3945175
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g632847 ELG44 Exostosin-like glycosyltransferase 44; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCCCGTTAATTACACGCAAATGCACCATCCTATCCCCTCCCATCGT
Internal bar code:AGTGGACCTGGGTCGTCTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:18.75
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCCAAACAGCCTCCAT
Suggested primer 2:AACACACACACAATCGCACG