| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078825 |
| Chromosome: | chromosome 12 |
| Location: | 9150502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543650 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAACCGAATCCCAGAGCTGGAGCGCGAGCCTCTTGGAGCCCGCGAAGC |
| Internal bar code: | TTGGACTTCCCCTACTGCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1495 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCATGAGCTCCCAATTGT |
| Suggested primer 2: | GGCCTGATAACGAGACCGAG |