| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078825 |
| Chromosome: | chromosome 12 |
| Location: | 9150508 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543650 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTATTCAGCGGGCCCGGTTGCTGGCTTTTTGGGGCCGCGGTGCGTTCC |
| Internal bar code: | GTTAGGGACTTGATAATCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1584 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTGATAACGAGACCGAG |
| Suggested primer 2: | TGGCATGAGCTCCCAATTGT |