| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078856 |
| Chromosome: | chromosome 6 |
| Location: | 2961294 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273650 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGCGCCTATAAGCGCCGAGCATAACTTTCCACGCCGCTGACATTGC |
| Internal bar code: | GTTCGGAAGTAAGGTCGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2916 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGGAACAACGCAAGAAA |
| Suggested primer 2: | GGTATGCTCAACCCCTTGCT |