Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.078873 |
Chromosome: | plastome |
Location: | 25119 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
CreCp.g802275 | rps19,ChreCp013,2717056 | 30S ribosomal protein S19; (1 of 1) K02965 - small subunit ribosomal protein S19 (RP-S19, rpsS) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATATATAAACTCTAAGGATAAACAAAACGAACCAATCAGTTGAAACAA |
Internal bar code: | ATTTAGTGAAAAGTTAGATCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 171 |
LEAP-Seq percent confirming: | 61.9048 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCGTCCAGTTACACCATG |
Suggested primer 2: | GAGGATGGTAGTGGCAGCTC |