| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078874 |
| Chromosome: | chromosome 17 |
| Location: | 5757494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741000 | (1 of 1) K13249 - translocon-associated protein subunit alpha (SSR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTACGCTGACAGCCTCTCTACCTCTGCGACAGCAGCACGCGCACTA |
| Internal bar code: | TCTGTGGGTTGCTATGCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5100 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTGCCTCTTGTGAAGA |
| Suggested primer 2: | CCCATCTATGCAATTGCCGC |