Insertion junction: CLIP2.078888_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.078888
Chromosome:chromosome 11
Location:1275326
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGTCCACGGATGAGCACCAGAGGCAACCGCAGAGAAAATGCGGACAGA
Internal bar code:AAAACTTGTACAAAGTTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:232
LEAP-Seq percent confirming:21.4286
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGACAGTGGTAAGGCAGGTT
Suggested primer 2:CAGCACAGTTGTCAACAGCC