| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078904 |
| Chromosome: | chromosome 3 |
| Location: | 6414697 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192850 | (1 of 1) 2.3.1.193 - tRNA(Met) cytidine acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCCCACCCTCGCCGCCTCCGCCCCTGCCGCCCAGCCCCTTGCCACCG |
| Internal bar code: | AGACTTCGCTTGCGGGCAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATGTGTGGGCCAGTGTG |
| Suggested primer 2: | GGACTTTGCGGTACCCTTCA |