| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.078941 |
| Chromosome: | chromosome 17 |
| Location: | 3627641 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725301 | (1 of 3) IPR000504//IPR003954 - RNA recognition motif domain // RNA recognition motif domain, eukaryote | 5'UTR | |
| Cre17.g725350 | PSL4,GLC2B | Glucosidase IIb; (1 of 1) K08288 - protein kinase C substrate 80K-H (PRKCSH) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGATTGTGCCCCAACTGTGCCGATCGGGGGCATCCCCGCCCCGTCTT |
| Internal bar code: | CCTATGATTACTGGGAAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2179 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGTCCTTCATCACGCC |
| Suggested primer 2: | ATCTTTCGGTTCCACACGCT |