Insertion junction: CLIP2.078948_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078948
Chromosome:chromosome 13
Location:5137104
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g607100 CYG6 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCACACACACGCACACACACACACGCACACACACACACACACACACA
Internal bar code:GCGGGTTGCATGGTTTTTGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:258
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCGTCGCATTTGAACAA
Suggested primer 2:CGTTGGTCGAAAAGCCGATC