Insertion junction: CLIP2.078963_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.078963
Chromosome:chromosome 5
Location:1156423
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g248550 (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTGTCAGTGCCCAGACGACCTGGGTCCGCGTTCCCACACGCTTCGACG
Internal bar code:TATAGCCATGGAGGTTTCTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1606
LEAP-Seq percent confirming:95.082
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTGCAGCAGTGGTGTTG
Suggested primer 2:AATGTGTGTGTTTGCGGACG