Insertion junction: CLIP2.078966_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.078966
Chromosome:chromosome 7
Location:4313178
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g341300 XAP5 (1 of 1) K13119 - protein FAM50 (FAM50, XAP5); transcription factor controlling ciliary gene expression 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGTGCAGATCAAGGACCGCACGCTTCAGCAGCTCAGGACCAGGGCTT
Internal bar code:CCTGACCAGCGGTTCCAACACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1256
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGATGATGGTGGTGCTC
Suggested primer 2:GCTGAGGGCCCTGAATTCTT