| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.078968 |
| Chromosome: | chromosome 3 |
| Location: | 397306 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144807 | MAS1 | (1 of 1) 2.3.3.9 - Malate synthase / Malic-condensing enzyme; Malate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCAGCGACAAGCAGCGGCGGTCAGCAAGGCGCTTCAAGTTAGCGCC |
| Internal bar code: | CATTCGATCAGGTTTGGGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGTCCTTTGACCAGGAA |
| Suggested primer 2: | CTGCATGATTGGGGACTGGT |