| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.078985 |
| Chromosome: | chromosome 10 |
| Location: | 6620619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465900 | CDKA1 | Cyclin-dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAGTAACCTGGAGAGCACCCTGCTAGAAGTCGAGTGAGGGTCGGAG |
| Internal bar code: | GCTACCTGATGAGGTTAGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2179 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAGCACGTCGTAGAGCC |
| Suggested primer 2: | TGATTCGAGGGGCTAGACCA |