Insertion junction: CLIP2.078990_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.078990
Chromosome:chromosome 16
Location:5336189
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g683650 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTGATCCCACAATTCCAGCGACTTTTGCGACCGGCTCTCCACCGACC
Internal bar code:GCCGCGCTGGGGGGCACCTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:815
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGTCACTTGATCCCACA
Suggested primer 2:GAGATGTGCCGCTCTACCTC