| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078995 |
| Chromosome: | chromosome 16 |
| Location: | 3003165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663850 | SSS5 | Soluble starch synthase V; (1 of 2) PTHR12526//PTHR12526:SF304 - GLYCOSYLTRANSFERASE // STARCH SYNTHASE 4, CHLOROPLASTIC/AMYLOPLASTIC-RELATED | 3'UTR |
| Cre16.g663876 | (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGACCGGACGTCACGTGTCGCGTCGAGCCTGTAAATTAACAAGAAAAG |
| Internal bar code: | TACTTGGTTGAGCGAGGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4083 |
| LEAP-Seq percent confirming: | 55.102 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTTGTTGACTTGGTGC |
| Suggested primer 2: | GCAATGTGCAGGTGTTCGTT |