| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.079045 |
| Chromosome: | chromosome 10 |
| Location: | 4084573 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448200 | ARL9 | ARF-like GTPase; (1 of 4) K07937 - ADP-ribosylation factor 1 (ARF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCATGCAGTCCTCACGGCCCATGTCTCGATCGCTTCTCCTGCACCTA |
| Internal bar code: | TCAAGGTAGTGATTAAGACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 26.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCCTGCGTGTAGGTAGA |
| Suggested primer 2: | TAGTAGGCACAGCAAACGCA |