| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079052 |
| Chromosome: | chromosome 16 |
| Location: | 5957043 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCACTGTGCGCGGAGTCACACGGACTTTGTCCGCGGGAGGGTTTGCA |
| Internal bar code: | TCTTACCGAGGTCGTCACGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1972 |
| LEAP-Seq percent confirming: | 51.8519 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTGCTCTTACGGGCTG |
| Suggested primer 2: | CTCCTTATGTTCGCTTGCGC |