| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079053 |
| Chromosome: | chromosome 9 |
| Location: | 4599741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801059 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCAAGACATTGTTCGCCCGGTTTTGGGGCGGGTTTTGTCCCAATTCC |
| Internal bar code: | ACGCTGATTCTCAGTTTATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2442 |
| LEAP-Seq percent confirming: | 36.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTACCACGACCACAGCAG |
| Suggested primer 2: | TGTGTGTGTGTGTGTGTGGA |