| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079056 |
| Chromosome: | chromosome 2 |
| Location: | 2801307 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094300 | FUO1 | (1 of 1) 1.5.5.1 - Electron-transferring-flavoprotein dehydrogenase / ETF-ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCCATCCGTGACAAGCATGCGAGTGACGTGGGAATGTGTGCGGATC |
| Internal bar code: | CGATTCTCGGAAAGAACGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1835 |
| LEAP-Seq percent confirming: | 59.4595 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCCAACGCAAAGCACT |
| Suggested primer 2: | GATGTCCTTTCCAGCAGCCT |