| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.079059 |
| Chromosome: | chromosome 2 |
| Location: | 6755518 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387726 | AST1 | Aspartate aminotransferase; (1 of 1) K14454 - aspartate aminotransferase, cytoplasmic (GOT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTCAATGTGTATGTGAAATTGTGTGTGCAGGCCTCAGATGCTGCTGT |
| Internal bar code: | GTAACTGGAAGGGTTATGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCAACGTCACAGCACGA |
| Suggested primer 2: | GGAGCACAAGGAGAACGGAA |