| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.079068 |
| Chromosome: | chromosome 1 |
| Location: | 2647592 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016001 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCAATCATTGGCTCTGAAGGCCTCCAGCGCCCCAACTCAGCTGCCGC |
| Internal bar code: | GCGACGGTCCTGCTTACTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1237 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTTGAACTACGGGAG |
| Suggested primer 2: | CTAGGCCGACATGTGTGTGT |