| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079074 |
| Chromosome: | chromosome 1 |
| Location: | 7836952 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055400 | (1 of 1) PF05022 - SRP40, C-terminal domain (SRP40_C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCAGTAACACCTCAAAGAGGCCAAGGCACGCCCTCCCAAACGCAGC |
| Internal bar code: | GGTGATAGGAGCTGAGTCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 26.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAAATGAGTGTGGCGAG |
| Suggested primer 2: | GAAGGCCACGTTGCACAATT |