| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.079081 |
| Chromosome: | chromosome 10 |
| Location: | 6228532 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463150 | DCI1 | Enoyl-CoA hydratase/isomerase family; (1 of 1) PTHR11941//PTHR11941:SF43 - ENOYL-COA HYDRATASE-RELATED // DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCATCATGCTCTCCTCACGCTGTCCACGCCTGCAGGGACAGCCCGCG |
| Internal bar code: | GTAATTGCTAGTGTTGCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGACCGAGAGCATGAGA |
| Suggested primer 2: | GTTGGGGGTGGGATTAGTGG |