Insertion junction: CLIP2.079082_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.079082
Chromosome:chromosome 8
Location:1486893
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g364800 (1 of 1) 6.3.5.3 - Phosphoribosylformylglycinamidine synthase / Phosphoribosylformylglycinamidine synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTGCGCACACTCGAAGGCGTGTCCTTCCAACGTGTCCTTCCAATGAC
Internal bar code:GCTGTTGTTTTGCGCGGGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:273
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCCGGACATCCCACAAC
Suggested primer 2:CCACCACCACCTTACTCCAC