Insertion junction: CLIP2.079101_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.079101
Chromosome:chromosome 17
Location:3553279
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724850 (1 of 1) K14848 - ribosome assembly protein RRB1 (RRB1, GRWD1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAGCACGCTGGACGCAGTTCGACGCCCACGAGAGGCGCAGGGGCGCG
Internal bar code:ACTGAGGATAATGGGTTTCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:5128
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACATACGGACACGCACT
Suggested primer 2:CGGCAGACAGTTGTTGTTGG