Insertion junction: CLIP2.079106_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.079106
Chromosome:chromosome 7
Location:3623367
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g337050 (1 of 1) K13346 - peroxin-10 (PEX10) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGCGTAACGCAACCAAGAATGATAAGTGCCGCGCTGTCATGTCTCGC
Internal bar code:CGATCGGCAGGGGGGTTCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1139
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGATGCGTGATAGGCCGA
Suggested primer 2:CGCTGTACCACACAAACTGC