| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.079107 |
| Chromosome: | chromosome 6 |
| Location: | 62999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249150 | MAPKK1 | Mitogen-Activated Protein Kinase Kinase 1; (1 of 1) PTHR24361//PTHR24361:SF408 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE STE7 | 5'UTR |
| Cre06.g249300 | ARP5,ARP4 | (1 of 1) K11340 - actin-like protein 6A (ACTL6A, INO80K); Actin-related protein, putative SWR-C component | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTGCGCAGCTTGTATTTACCGCTTTTCGCTGCCTCCACATACAGTA |
| Internal bar code: | GTCTATGGGAGCCTAGCGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 1.36986 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCACCTCCATCCCGATT |
| Suggested primer 2: | TCAAAGCCACTCACGATGCT |