| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079114 |
| Chromosome: | chromosome 9 |
| Location: | 93634 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386400 | FAP124,UBA1,POC20 | (1 of 1) K03178 - ubiquitin-activating enzyme E1 (UBE1, UBA1); Ubiquitin E1 activating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAACTCACAGCCCAGCGCGCCAGCACCCACCTGCAGGGGAGGAGGCAG |
| Internal bar code: | GTTGCATTCCATCGCCTGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTTAAGGCTCACCCATG |
| Suggested primer 2: | CACACACTCCTCAATCCCCC |