| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.079118 |
| Chromosome: | chromosome 2 |
| Location: | 3338056 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097900 | AST3 | (1 of 1) K00811 - aspartate aminotransferase, chloroplastic (ASP5); Aspartate aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCTCCCAAGAGCACGTTTCCTCGCCAAGAGGAAGCGTTGTGGGGCG |
| Internal bar code: | CGCGAGTTCACATTATGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGGATACTCGACCGCTC |
| Suggested primer 2: | TCCCAGGAAAGGCTATCCCA |