| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.079120 |
| Chromosome: | chromosome 4 |
| Location: | 638878 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217945 | FAD5D | Putative Linoleate desaturase; (1 of 2) K00507 - stearoyl-CoA desaturase (delta-9 desaturase) (SCD, desC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCCCGACCCCACCGTGTGCTGTTGAGCGCCTCATCGAACAGCCAGCC |
| Internal bar code: | CTTGAGGCTGTCTTGGCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCCAAACTTGCGAGT |
| Suggested primer 2: | CGTCGTCACTCACTCACCAA |