| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079132 |
| Chromosome: | chromosome 4 |
| Location: | 2300508 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220700 | DIV43,ALK2 | Aurora-like kinase; (1 of 1) IPR030616 - Aurora kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTGTCTTTGAGGCCGTGCCTTGATGCCTTTGGATACTTCGTACTCC |
| Internal bar code: | GGAATTGCACAGGCCGCTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1094 |
| LEAP-Seq percent confirming: | 9.09091 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTCAACACTTGGCCAGC |
| Suggested primer 2: | CTTGAAGCTGGGTGCAACAC |