| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079141 |
| Chromosome: | chromosome 9 |
| Location: | 81659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386250 | SMM33 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTGCGCAAGAACTGGCAGTTGGCTGCCTGCATCCCTCGGATTGTGAA |
| Internal bar code: | GGCTAATCAAACTTGTCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2588 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGATACACGGCATGCTAC |
| Suggested primer 2: | TGCTATCAGGGCCCAACTTG |