| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079142 |
| Chromosome: | chromosome 7 |
| Location: | 2894932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332500 | AXT2 | (1 of 3) PTHR10994:SF69 - PROLINE-RICH PROTEIN 18; Arabinose chain extension enzyme for HRGP 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCATGTGTTTTAGGAGTTAGGACGTTTGCTGTGAGCACTACCGCTGG |
| Internal bar code: | TGGCGACGGACGAGAGTGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2260 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGAAGGATGGGGCAGG |
| Suggested primer 2: | ACAACACGACATAGCAGCCA |