| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079148 |
| Chromosome: | chromosome 6 |
| Location: | 7894393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303850 | MRPL114,mL114 | mitochondrial ribosome protein mL114, Chlamydomonas-specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCACTACCGTAATGCCTAGACAGCAACAAAACGGCACAAACTTGCAC |
| Internal bar code: | TCCGGGATTTTCGTCTAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1899 |
| LEAP-Seq percent confirming: | 68.5714 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCACTACGAGCTTGAC |
| Suggested primer 2: | GCAAACACTCTGTGTGGCAG |