| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079156 |
| Chromosome: | chromosome 9 |
| Location: | 4522420 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400960 | (1 of 1) IPR006311//IPR007657 - Twin-arginine translocation pathway, signal sequence // Glycosyltransferase AER61, uncharacterised | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCATTGTCGTGTTGCTGCCTTGCTGCATTCCTATGTATGTGTTTA |
| Internal bar code: | AGGGCAGTATTGCCGGGATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2424 |
| LEAP-Seq percent confirming: | 87.8788 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCGTTGTGGTAGAGCT |
| Suggested primer 2: | CCCGACTCAGGAAAAGGCAT |