| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079156 |
| Chromosome: | chromosome 9 |
| Location: | 4522436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400960 | (1 of 1) IPR006311//IPR007657 - Twin-arginine translocation pathway, signal sequence // Glycosyltransferase AER61, uncharacterised | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGAGTCGCCATCCGCGGTCGAAAGCTTCCTCCAAAGCTGCCTAGTAC |
| Internal bar code: | GGCCAAAATTGAATATAATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1407 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGACTCAGGAAAAGGCAT |
| Suggested primer 2: | GAAGGCGTTGTGGTAGAGCT |